Analysis of Sildenafil Citrate in Herbal for Men and Herbal for Stamina Enhancers Using High Performance Liquid Chromatography

Jamu is a traditional Indonesian medicine that has been used for generations based on experience, using raw materials that have not been standardized. In accordance with existing laws and regulations, traditional medicines are prohibited from adding isolated chemicals or synthetic compounds with medicinal properties, known as medicinal chemicals (BKO). Sildenafil citrate is one of drugs that are often added to herbal medicine samples. The purpose of adding sildenafil citrate for “jamu” for men and stamina enhancers is to improve the quality and duration of consumer erections. The purpose of this study was to conduct a qualitative and quantitative analysis of sildenafil citrate in herbal medicine samples for men and stamina enhancers that are circulating in the Magelang area, Central Java. The method chosen was thin layer chromatography with a stationary phase of silica gel F254 with two different mobile phase systems, namely methanol: chloroform (1:1) and (4:1) and high-performance liquid chromatography for quantitative analysis with a stationary phase of a C18 column and a mobile phase of methanol: acetonitrile (70:30) with a flow rate of 1 ml/min. The results of the study showed that 1 in 3 herbal medicine samples contained sildenafil citrate with an average level of 0.75%.