PEDIOCIN PRODUCTION BY PEDIOCOCCUS ACIDILACTICI IN CONTINUOUS MODE USING MEAT PROCESSING WASTE

Objective: The production of pediocin by Pediococcus acidilactici has been studied on a bioreactor in continuous mode. Meat processing waste (MPW) has been used as a growth medium. Different dilution rate has been attempted in continuous process to maximize the pediocin production. Methods: The continuous fermentation process was carried out at various dilution rates, ranging from 0.075 h-1 to 0.15 h-1. A mathematical model has been developed for simulation of the continuous process. Results: The maximum pediocin activity was observed 2743 AU/ml in continuous cultures at dilution rate of 0.075 h-1. The values of correlation coefficient (R2) were in the range of 0.94 to 0.98. The predicted model was well validated. Conclusion: The continuous pediocin production could be possible for large-scale application.