Integration of single-cell RNA and bulk RNA sequencing revealed malignant ductal cell heterogeneity and prognosis signatures in pancreatic cancer
Our integrative single-cell and bulk-RNA workflow identifies ANLN, NT5E, and CTSV as novel prognostic biomarkers in pancreatic cancer and highlights a pro-tumorigenic interaction between malignant ductal cells and macrophages. Targeting CTSV or disrupting CXCL14-CXCR4 and IL1RAP-PTPRF signaling may offer new therapeutic avenues for PAC.
Authors
- Xuejie Song (ORCID: https://orcid.org/0009-0009-1325-1793)
- Fuchun Si (ORCID: https://orcid.org/0000-0002-9880-7333)
- Gao Si (ORCID: https://orcid.org/0000-0002-9583-1928)
- Jiqing Si
- Haiyang Du
Institutions
- Henan University of Traditional Chinese Medicine (CN)
- Peking University (CN)
- Peking University Third Hospital (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-07-01
- DOI
- https://doi.org/10.3389/fimmu.2025.1579184
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Education Department of Henan Province
- Henan Provincial Science and Technology Research Project