Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat
The present manuscript is first of its kind to address the holistic approach, starting from efficient gene selection, gRNA designing, and post-gRNA designing issues like gRNA stability, binding efficiency, and functionality for SDN1-CRISPR/Cas9 genome editing in wheat. This manuscript will be a ready reference for wheat researchers designing effective gRNA for wheat improvement to meet future food demand.
Authors
- Tushadri Singh (ORCID: https://orcid.org/0009-0001-0582-7096)
- Zeenat Wadhwa
- Singh Rajender (ORCID: https://orcid.org/0000-0002-4592-6566)
- Om Parkash Ahlawat (ORCID: https://orcid.org/0000-0002-9225-3763)
- Omvir Singh (ORCID: https://orcid.org/0000-0003-3594-4215)
- Rakesh Kumar (ORCID: https://orcid.org/0000-0003-0811-5657)
- Ratan Tiwari
- J.P. Jaiswal
- H. M. Mamrutha
Institutions
- Govind Ballabh Pant University of Agriculture and Technology (IN)
- Indian Institute of Wheat and Barley Research (IN)
Publication Details
- Journal
- Frontiers in Genome Editing
- Published
- 2025-07-01
- DOI
- https://doi.org/10.3389/fgeed.2025.1579165
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00