Evaluation of the effects of G4 ligands on the interaction between G-quadruplexes and their binding proteins
G-quadruplexes (G4s) regulate multiple biological processes by interacting with G4-binding proteins (G4BPs). We developed a sandwich ELISA system to evaluate G4 ligand effects on G4-G4BP binding. G4 ligands strongly affect G-quartet-protein interactions, demonstrating that high binding affinity of G4 ligands for G4s does not guarantee ternary complex formation with G4BPs.
Authors
- Daimei Miura (ORCID: https://orcid.org/0009-0007-0005-9976)
- Hiroyuki Sasanuma (ORCID: https://orcid.org/0000-0003-2824-4499)
- Masayuki Tera (ORCID: https://orcid.org/0000-0003-4430-579X)
- Ryutaro Asano (ORCID: https://orcid.org/0000-0001-6795-8377)
- Hisao Masai (ORCID: https://orcid.org/0000-0003-1268-5302)
- Kazuo Nagasawa
- Yuma Tanaya
- Manaho Sashida
Institutions
- Tokyo Metropolitan Institute of Medical Science (JP)
- Tokyo University of Agriculture and Technology (JP)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc02801a
- Citations
- 4
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 3.22