Molecular Study and Detection of The Causative Bacteria of Urinary Tract Infection of Transfusion Dependent Thalassemia Patients Who Are Being Treated with Iron Chelators

This study was conducted for the purpose of detecting some virulence factor genes of the bacterial species isolated from the urine transfusion dependent beta Thalassemia TDT with UTI, 173 urine samples were collected from both study group TDT patient with UTI and control group, Results showed that sample (n=38, 21.96%) of the total samples studied gave positive bacterial growth, while (n=135, 79.03%) gives negatives growth on media cultivation in control samples. In patient with beta Thalassemia have shown positive growth of 38 samples (21.96%) and samples (135) (79.03%) were not give any growth. the purpose of study is to detecting some virulence factors for gram negative studied isolates, DNA was extracted for all isolates. (n=17) gram negative isolates were taken, represented by (n=9, 23.7%) isolates of E. coli and (n=3, 7.9%) isolates of E. cloacae, (n=5, 13.2%) of K. pneumonia their isolated from transfusion dependent thalassemia with UTI with use of universal primers for each bacterium and partial 16s rRNA sequence for diagnosis of this isolated bacteria. Highlights: Detecting virulence factor genes in bacteria from transfusion-dependent beta thalassemia UTI patients. 173 urine samples, 38 (21.96%) showed positive bacterial growth, 135 negative. DNA extraction, 17 gram-negative isolates identified: E. coli, E. cloacae, K. pneumoniae. Keywords: universal primers, Thalassemia, Urinary tract infection, Virulence Factors.