Testing of vaccine against canine distemper, parvovirus and coronavirus enteritis, adenovirus infection and dog rabies for its antigenic properties

Introduction. Recently “Carnican-5R” vaccine against canine distemper, parvovirus and coronavirus enteritis, adenovirus infection and dog rabies has been developed at the Rosselkhoznadzor-subordinated Federal Center for Animal Health (FGBI “ARRIAH”, Vladimir) in accordance with the Russian Federation legislative requirements. The virus strains currently circulating and significant in the country were used for the vaccine development. Objective. Testing of “ Carnican-5R” vaccine for its antigenic properties in target animals including determination of humoral immunity development time and duration during the observation period. Materials and methods. “Carnican-5R” combined vaccine containing two components: freeze-dried component and liquid component were used for the test. Dogs at the age of 10–12 weeks served as animal models for testing the vaccine for its antigenic properties. The antibody levels were determined with virus neutralization test, hemagglutination inhibition test and fluorescent antibody virus neutralization test. Results. Vaccination of dogs was found to induce antibodies to the pathogens of the specified infections. Double “Carnican-5R” vaccine administration at 21-day interval induced strong humoral response by day 35 after its first administration and an increase in the antibody titers to canine distemper – by 8.6 times, to canine parvovirus type 2 – by 2.1 times, to canine coronavirus – by 5.0 times, to canine adenovirus serotype 2 – by 5.36 times, to the rabies virus – by 5.72 times. The specific immunity lasted for at least 12 months and virus-specific antibodies titers to the pathogens remained at the protective levels. Conclusion. “Carnican-5R” vaccine is safe and non-reactogenic for target animals and induces strong immunity in dogs that lasts for at least 12 months from the date of booster vaccination.