Transcranial Magneto-Acoustic Stimulation Enhances Cognitive and Working Memory in AD Rats by Regulating Theta-Gamma Oscillation Coupling and Synergistic Activity in the Hippocampal CA3 Region

<b>Background:</b> Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive dysfunction and working memory impairment, with early hippocampal damage being a prominent feature. Transcranial magneto-acoustic stimulation (TMAS) has been shown to target specific brain regions for neuroregulation. <b>Methods:</b> This study investigated the effects of TMAS on cognitive function, working memory, and hippocampal CA3 neural rhythms in AD rats by specifically stimulating the hippocampal region. <b>Results</b>: The novel object recognition test and T-maze test were employed to assess behavioral performance, while time-frequency analyses were conducted to evaluate memory-related activity, neural synchronization, and cross-frequency phase-amplitude coupling. TMAS significantly improved cognitive and working memory deficits in AD rats, enhancing long-term memory performance. Additionally, the abnormal energy levels observed in the θ and γ rhythm power spectra of the CA3 region were markedly restored, suggesting the recovery of normal neural function. This improvement was accompanied by a partial resurgence of neural activity, indicating enhanced inter-neuronal communication. Furthermore, the previously damaged coupling between the θ-fast γ and θ-slow γ rhythms was successfully improved, resulting in a notable enhancement of synchronized activity. <b>Conclusions:</b> These findings suggest that TMAS effectively alleviates cognitive and working memory impairments in AD rats and may provide experimental support for developing new treatments for AD.