Anti-Atherosclerotic Activity of Plumeria Acuminata Leaf Extract Against Triton X-100 Induced Hyperlipidemia Model in Rats

Background amp Objectives Hyperlipidemia characterized by elevated levels of lipids in the blood is a significant risk factor for cardiovascular diseases including atherosclerosis. This study explains the potential effects of the methanol extract of Plumeria acuminata leaves against atherosclerosis.Methods Nine formulations of mucoadhesive microspheres were created incorporating Carbopol-934P and different ratios of AIFM. A central composite design was employed using Design-Expert software to assess the influence of independent variables concentrations of polymers on the dependent variable mucoadhesive strength. The microspheres underwent compatibility studies and various physical evaluations including analysis of Acyclovir content and its discharge kinetics. Anti-atherosclerotic activity of P. acuminata was evaluated on Triton X-100 induced hyperlipidemia in rats by measuring levels of biochemical serum parameters and histopathological assessment. Results The in-vivo anti-atherosclerotic activity of different doses 100 200 400 mgkg of methanol extract of P.acuminata leaves was carried out against Triton X-100 induced hyperlipidemia model. During the study it was found that the methanol extract of P.acuminata leaves MEPAL resulted in significant Plt 0.001 decrease in the biochemical serum parameters such as ALT AST ALP total cholesterol Triglycerides LDL Cholesterol HDL-Cholesterol LDH VLDL-Cholesterol CPK and FFA levels as compared to disease control groups and these findings were further supported with histopathological studies. Thus the present finding provides scientific evidence of ethnomedical value of P.acuminata leaves in cardiovascular diseases.Conclusion Methanolic extract of P.acuminata leaves possess a significant anti-atherosclerotic activity against Triton X-100 induced hyperlipidemia in Albino Wistar rats