Evaluation of the effect of BAP, Kinetin, Meta-Topolin growth regulators on grape cultivars and molecular analysis of SSR markers

Preserving genetic similarity is one of the key points to enhance resilience, functionality, and unique adaptation. Micropropagation is the most reliable approach to preserving genetic similarity. In this work, we compared the efficiencies of the three modified growth culture media – Murashige and Skoog (MS), Woody Plant Medium (WPM), and Driver-Kuniyuki Wallnut (DKW) on shooting number and length. The efficiency of BAP, kinetin, and meta-topolin in the above culture media has been established for four local Uzbek grape (Vitis vinifera L.) cultivars. MS was found to be the most efficient culture media among the studied ones to grow Odqum kishmish, Rizamat, Toyfi, Sugdiyona kishmish grape cultivars, belonging to highly productive ones in Uzbekistan. The numb er of shoots in the studied cultivars increased by 8–10 when grown In vitro in the modified MS culture media compared to the unmodified one. Significant improvements were observed in the shoot length in all grape cultivars when grown in modified MS media. Using eight Simple Sequence Repeat markers, we determined the preservation of genetically identical material of in vitro grown Oqdum kishmish explants with the highest parameters. In this work, grape microplants were in vitro grown in modified culture media and were widely used in research. MS was established to be optimum for all four cultivars. We also determined that culture-grown Oqdum kishmish micropropagations were genetically similar to mother plant.