Anti-Lung Cancer Activity of the Herbal Medicinal Plant <i>Cycas revoluta</i>

Background: Phytochemicals have demonstrated strong potential in cancer therapy, and several researchers became interested in evaluating the anti-cancer effects of Cycas revoluta, a plant in herbal medicine. Objectives: The present study examined the anti-proliferative effect of the n-butanol fraction from Cycas revoluta root extract on A549 human lung adenocarcinoma cells. Materials and Methods: The roots were defatted with n-hexane and then extracted. The reflux technique—Soxhlet extraction with 85% ethanol and 15% distilled water—was then used to obtain the crude extract of the root, which was subsequently fractionated using the solvents chloroform, ethyl acetate, and n-butanol, respectively. Cytotoxicity was evaluated using the MTT assay after 72 hours of treatment. Results: Initial results indicate that the n-butanol fraction has potential as an anti-cancer agent for lung cancer cells and merits further investigation into its mechanisms and active compounds. The present work adds to the increasing evidence of plant-based anti-cancer agents and the potential of C. revoluta in lung cancer therapy. Conclusion: The n-butanol extract from the roots of C. revoluta exhibits notable anti-proliferative effects on A549 lung cancer cells, indicating its potential as a natural anti-cancer therapeutic agent. Additional research is required to identify the active components, clarify the molecular mechanisms involved, and assess the in vivo effectiveness.