Assessment of the Isolation of Bioactive Molecules from the Root Extract of Nardostachys Jatamansi

Introduction: Nardostachys jatamansi, a perennial herb renowned for its therapeutic properties in traditional medicine, has been the focus of extensive research due to its potential pharmacological significance. Objective: This study aimed to isolate and characterize sesquiterpene compounds from the root extract of N. jatamansi. Methods: Chromatographic techniques were used in a stepwise manner for solvent extraction, and isolation process. The isolated sesquiterpene was subjected to various spectroscopic analyses, including UV-visible spectroscopy, nuclear magnetic resonance (NMR) and mass spectrometry (MS), to elucidate its structural composition and confirm its purity. Result: Results indicated that the Nardostachys jatamansi and Std. Sesquiterpenoid Rf values were determined to be 0.53 and 0.53, respectively. For column chromatography, toluene: ethyl acetate: acetic acid (9:1:0.2) was chosen as the mobile phase. Using mass spectroscopy, a mass spectrum of the isolated component (Fraction H) of the alcoholic extract from Nardostachys jatamansi was captured. The successful isolation of a sesquiterpene compound from N. jatamansi root extract, with spectral data supporting its structural identification. The elucidation of the sesquiterpene's chemical structure contributes to the understanding of the plant's chemical composition and potential therapeutic applications. Conclusion: This research provides a foundation for further investigations into the pharmacological properties of N. jatamansi and its constituents, potentially paving the way for further novel pharmaceutical evaluation. DOI: https://doi.org/10.52783/jchr.v15.i3.8391