Application of Oral Mucosal Epithelial Cells in Noninvasive Pathological Diagnosis of Neuronal Intranuclear Inclusion Disease
We employed a novel method for collecting oral mucosal epithelial cells, an anagar-paraffin double-embedding technique for cell fixation in cytopathological processing. OMEC biopsy demonstrated a high diagnostic efficacy for NIID: H&E staining revealed inclusions in 60% (12/20) of patients, TEM confirmed them in 40% (8/20) of cases and immunofluorescence detected p62-positive aggregates in 9/20 (45%) and uN2CpolyG in 8/20 (40%) of cases, respectively. OMEC biopsy is a simple, noninvasive technique for detecting intranuclear inclusions in NIID, avoiding the need for invasive surgical procedures such as skin or labial gland biopsies.
Authors
- Ying Ji
- Zichang Yin
- Ming Shi (ORCID: https://orcid.org/0000-0001-9263-6468)
- Hao Chen (ORCID: https://orcid.org/0000-0003-0393-723X)
- Jin Tian (ORCID: https://orcid.org/0000-0002-5202-7796)
- Xianhong Jia
- Yu Kong
- Wenjing Song (ORCID: https://orcid.org/0000-0001-8696-043X)
- Xiaowen Li (ORCID: https://orcid.org/0000-0003-1571-034X)
- Lei Bao (ORCID: https://orcid.org/0000-0002-7496-7340)
- Jingwen Yang (ORCID: https://orcid.org/0000-0002-8206-2584)
- Xingxing Yuan (ORCID: https://orcid.org/0000-0002-9894-4127)
- Qingqing Ji (ORCID: https://orcid.org/0000-0002-5549-949X)
- Yuwen Li (ORCID: https://orcid.org/0009-0000-9359-8416)
- Xiuhui Sun
Institutions
- Xuzhou Medical College (CN)
- Kingmed Diagnostics (CN)
Publication Details
- Journal
- Neuropathology and Applied Neurobiology
- Published
- 2025-06-30
- DOI
- https://doi.org/10.1111/nan.70023
- Primary Topic
- Genetic Neurodegenerative Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00