FEATURES OF BONE WOUND REGENERATION USING OSTEOPLASTIC MATERIALS

The aim of the study was to microscopically evaluate the features of bone regeneration in an infected experimental wound using osteoplastic materials and their combined use with a two-layer membrane “Hyprosorb F”. Material and methods of the study. The experiment was conducted on 135 rats. To create an experimental bone wound, a milling hole was applied to the area of the lower jaw. “Collapan-L” (group I) or “Collapan-L” with a two-layer membrane “Hypro-sorb F” (group II) was placed in the bone wound on the left side. The experimental wound was sutured without any material (control). After the animals were withdrawn from the experiment, a section of bone adjacent to the experimental wound was isolated and microscopically examined. Results of the study. On the 10th day of the experiment, osteoblastic granulation tissue with its maturation into cancellous bone was observed in animals of group I. In group II, the injected stem cells reduced the severity of the inflammatory reaction to foreign material in the defect area and accelerated the formation of bone tissue along its edge. On the 20th day, both groups showed similar changes: maturation of granulation tissue with the formation of bone tissue and a decrease in inflammatory infiltration in the regeneration zone, especially by polymorphonuclear leukocytes. On day 30, both groups showed signs of granulation tissue maturation with the formation of spongy bone, which filled most of the bone defect. In comparison between study groups I and II, a less pronounced inflammatory reaction to the injected osteoplastic material, a greater specific volume of bone beams and bone marrow spaces were found with the joint use of osteoplastic material with a two-layer membrane “Hypro-sorb F” (p˂0.05). When comparing the study groups with the control, it was found that in both groups I and II, regardless of the use of the two-layer membrane “Hypro-sorb F”, faster regeneration in the bone defect and a decrease in inflammatory infiltration were observed. Conclusions. The analysis of tissue sections of the experimental wound indicates an increase in the regenerative ability of the osteoplastic material “Collapan-L” compared to the control, where the wound was sutured without the material. Comparing the effectiveness of regeneration with the use of “Collapan-L” and “Collapan-L” together with the two-layer membrane “Hypro-sorb F”, a faster disappearance of signs of inflammation and the appearance of bone formation in the second group of the study was established.