Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene
African swine fever virus (ASFV) causes a highly contagious and lethal hemorrhagic disease and significantly threatens the pig industry. There is no commercially effective vaccine available currently, making the detection of ASFV critical for control and prevention. Previously, we established the CRISPR-LbCas12a and LwCRSIRP-Cas13a visual detections of ASFV, separately, targeting the structural p17 gene D117L. In this study, we performed the parallel detections of ASFV based on the conserved viral protease gene S273R using CRISPR-LbCas12a and CRISPR-LbuCas13a systems. Our results showed that both systems are able to specifically detect ASFV as low as two copies of the S273R gene, and effectively detect clinical samples with minimal DNA purification. The work promotes CRISPR-Cas systems for the application of on-site detection in the field.
Authors
- Nanhua Chen (ORCID: https://orcid.org/0000-0002-1909-8760)
- Hua Han (ORCID: https://orcid.org/0000-0002-1872-9700)
- Anjing Liu (ORCID: https://orcid.org/0009-0000-0128-1877)
- Wanglong Zheng (ORCID: https://orcid.org/0000-0002-0939-331X)
- Jianfa Bai (ORCID: https://orcid.org/0000-0002-0021-3036)
- Jianzhong Zhu (ORCID: https://orcid.org/0000-0002-7082-1993)
- Wenyu Hao (ORCID: https://orcid.org/0000-0003-1397-4253)
- Nengwen Xia (ORCID: https://orcid.org/0000-0002-9480-0185)
- Desheng Zhang (ORCID: https://orcid.org/0000-0001-9307-8736)
- Jia Luo (ORCID: https://orcid.org/0000-0003-4936-7586)
- Sen Jiang (ORCID: https://orcid.org/0000-0002-5074-8565)
- Juan Gu (ORCID: https://orcid.org/0000-0003-0139-1712)
- Meng Cui
Institutions
- Kansas State University (US)
- Yangzhou University (CN)
Publication Details
- Journal
- Animals
- Published
- 2025-06-27
- DOI
- https://doi.org/10.3390/ani15131902
- Citations
- 4
- Primary Topic
- Animal Disease Management and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 7.61
Funders
- National Natural Science Foundation of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions