Comparison of Physico-Chemical and Antioxidant Activity of Sargassum henslowianum J. Agardh by Different Extraction Methods

Sargassum henslowianum is a seaweed that shows promise for extracting valuable compounds used in various fields. This study focused on the extraction of nutrients from S. henslowianum to identify biologically active compounds and assess acetylcholinesterase inhibitory activity in different extracts. The seaweed was extracted using water and EDTA and the extracts were analyzed for active compounds and AChE inhibitory activity using Ellman’s spectrophotometric method. The results indicated that the water extract had higher levels of polyphenols, flavonoids and antioxidant activity, with IC50 values of 1977.54 µg mL–1 (DPPH) and 68.82 µg mL–1 (ABTS). The water extract also showed a MIC of 2.813 mg mL–1 against E. coli and S. aureus, while the EDTA extract had a MIC of 5.625 mg mL–1. This study highlights the potential of seaweed as a source of acetylcholinesterase enzyme inhibitors. Extracting high-quality compounds from seaweed could lead to new opportunities in the pharmaceutical and cosmetic industries, supporting sustainable development in the marine sector.