Synthesis, Characterization, in vitro Antimicrobial and Antiproliferative Potentials of Novel Carvacrol Based Schiff Base Derivatives

A series of novel carvacrol based Schiff base derivatives (5-Cl, Nap, 3,5-Dibr, DEAS and 5-Br) were synthesized and characterized through FT-IR, 1H NMR and 13C NMR spectroscopic methods. The FT-IR confirmed the characteristic peak of imine (–CH=N–) at 1619-1514 cm-1, while the NMR spectra confirmed the arrangement of aromatic and aliphatic protons and carbons with halogen substitutions. The synthesized Schiff Base derivatives had significant biological activity, particularly Nap, 5-Br, and 3,5-Dibr, which exhibited high cytotoxicity against the A-549 lung cancer cell line. Antimicrobial assay indicated a strong lethality in brine shrimp assays and acceptable antibacterial activity, especially for 5-Cl and 3,5-Dibr, with minimum inhibitory concentrations (MIC). These findings confirm the structural integrity and pharmacological significance of Schiff bases, highlighting their usefulness in the development of anticancer and antibacterial drugs.