Caspase mediated apoptosis induced by CPT@PLA micro-nanofibers in C2C12 cells: insights into probable mechanism

Cancer is a complex medical condition that results from a convergence of variables such as lifestyle, environment and hereditary. Majority of chemotherapeutic medications are synthetic derivatives of plants/compounds that are extracted from plants. An approach was developed to efficiently generate micronanofibers via e-spinning. The present investigation targets to report on the influence of camptothecin (CPT) on C2C12 and impact of e-spinning on integrity of prodrug CPT. For the first time, a composite solution comprising CPT and poly(lactic acid) (PLA) was electrospun to generate CPT@PLA micronanofibers. Using Scanning electron microscopy, Energy dispersive-X-ray spectroscopy, Confocal microscopy, Electron probe microanalyzer, X-ray diffraction analysis, the mat was physiochemically characterized. The cell counting Kit-8 test was used to assess cytotoxicity of C2C12. To better comprehend mechanism of cell demise, influence of CPT@PLA micronanofibers on caspases 3 and 7 were probed. SEM and XRD confirmed interaction of CPT with PLA and demonstrated that crystallinity of CPT dwindled following encapsulation. Amalgamation of CPT in PLA influenced morphology as well as size of fibers. Cytotoxicity and molecular results illustrate that PLA is non-cytotoxic; contrariwise CPT@PLA micronanofibers considerably suppressed C2C12 cells. This study highlights potential of CPT@PLA micronanofibers as a foundation for developing future anticancer therapy, suitable for medical and biomedical applications.