Effect of Octopus (Octopus sp.) Ink Extract on Haematological of the Catfish (Clarias sp.) Infected with Aeromonas hydrophila

The catfish (Clarias sp.) is a freshwater fish species with economic value, leading to widespread cultivation by fish farmers. The growing demand for catfish consumption has driven a shift from traditional to intensive farming methods. However, intensive farming often faces issues such as disease outbreaks, which can cause significant mortality among the farmed fish. A common disease affecting catfish is Motile Aeromonas Septicemia (MAS), caused by Aeromonas hydrophila, which produces exotoxins that damage the infected fish's surface. One preventive measure that can be applied is the use of immunostimulants. Octopus ink extract is a potential immunostimulant known to enhance the immune system of the catfish. This extract contains bioactive compounds, including alkaloids, saponins, and lysine, which are believed to boost growth. This study investigated the effects of octopus ink extract on the immune system of catfish infected with Aeromonas hydrophila. The experimental method involved five treatments with three replications: Treatment 1 (Control), Treatment 2 (40 ml/kg), Treatment 3 (80 ml/kg), Treatment 4 (120 ml/kg), and Treatment 5 (160 ml/kg). The research steps included extracting octopus ink, determining the Lethal Concentration 50 (LC50), preparing equipment and materials, supplementing octopus ink extract in the feed, applying treatments, challenging with A. hydrophila, and maintaining the fish. The parameters measured during the research included immune system parameters. The immune system parameters assessed were erythrocytes, leukocytes, leukocytes differential, and hematocrit. The results of the study showed that Treatment 5 (160 ml/kg) yielded the best outcomes for immune system function. Immune system improvements were indicated by the highest values for erythrocytes (2.56 x 10⁶ cells/mm³), leukocytes (3.91 x 10⁴ cells/mm³), and hematocrit (32.9%). The addition of octopus ink extract into the catfish feed significantly improves the immune system (erythrocytes, leukocytes, and hematocrit). The catfish (Clarias sp.) is a freshwater fish species with economic value, leading to widespread cultivation by fish farmers. The growing demand for catfish consumption has driven a shift from traditional to intensive farming methods. However, intensive farming often faces issues such as disease outbreaks, which can cause significant mortality among the farmed fish. A common disease affecting catfish is Motile Aeromonas Septicemia (MAS), caused by Aeromonas hydrophila, which produces exotoxins that damage the infected fish's surface. One preventive measure that can be applied is the use of immunostimulants. Octopus ink extract is a potential immunostimulant known to enhance the immune system of the catfish. This extract contains bioactive compounds, including alkaloids, saponins, and lysine, which are believed to boost growth. This study investigated the effects of octopus ink extract on the immune system of catfish infected with Aeromonas hydrophila.[U1] [RIA2] The experimental method involved five treatments with three replications: Treatment 1 (Control), Treatment 2 (40 ml/kg), Treatment 3 (80 ml/kg), Treatment 4 (120 ml/kg), and Treatment 5 (160 ml/kg). The research steps included extracting octopus ink, determining the Lethal Concentration 50 (LC50), preparing equipment and materials, supplementing octopus ink extract in the feed, applying treatments, challenging with A. hydrophila, and maintaining the fish. The parameters measured during the research included immune system parameters. The immune system parameters assessed were erythrocytes, leukocytes, leukocytes differential, and hematocrit. The results of the study showed that Treatment 5 (160 ml/kg) yielded the best outcomes for immune system function. Immune system improvements were indicated by the highest values for erythrocytes (2.56 x 10⁶ cells/mm³), leukocytes (3.91 x 10⁴ cells/mm³), and hematocrit (32.9[U3] [RIA4] %). The addition of octopus ink extract into the catfish feed significantly improves the immune system (erythrocytes, leukocytes, and hematocrit). [U1]I don't think your study shows growth data. I think your study only focuses on the immune response. [RIA2]We have revised it. [U3]You can add conclusions from the results [RIA4]We have added it.