Antidiabetic and Anti-Inflammatory Activity of Sea Cucumber (Holothuria sp.) Captured from Madura Island, Indonesia

Holothuria sp. is a fishery commodity that has a high economic value and is very easy to find in Madura waters but has not been widely used. The high nutrition and bioactive compounds contained can be potential as drug and functional food candidates. This study aimed to determine the morphological and morphometric characteristics, analyze the proximate content, protein profiling, and its potential as an anti-inflammatory and anti-diabetic agent in vitro. The stages of this research consisted of raw material preparation, morphometric and morphological analysis, chemical characterization, protein extraction, and screening for anti-inflammatory activity using the protein denaturation method and anti-diabetic by inhibiting the α-glucosidase enzyme. The study used a completely randomized design and was analyzed using ANOVA with the Dunken further test. Sea cucumbers Holothuria sp. has a physical appearance of a black cylindrical shape with a length of around 12.94cm. Holothuria sp. has a high nutritional content with a protein of 3.62% and the molecular weight of meat protein ranges from 172.05-38.22 kDa and viscera of 187.96-56.92 kDa. The highest potential anti-inflammatory activity in 10,000ppm hydrolysate was 98.83% and antidiabetic activity in 10,000ppm hydrolysate was 85.34%. Sea cucumbers originating from Madura waters have the potential to be developed in the fungsional food and medicine sectors.