Evaluation of the Monocyte Chemotactic Protein-3 Gene (rs17735770) Polymorphism in patients with Type 2 Diabetes Mellitus

Background: Type 2 diabetes mellitus (T2DM) is a chronic and complex metabolic disorder, typically resulting from the progressive failure of insulin to regulate blood glucose levels. The chemokine monocyte-chemotactic protein-3 (MCP-3) gene polymorphism plays an important role in the pathogenesis of T2DM, its involvement in inflammatory processes and vascular complications that cause damage to pancreatic cells. Objective: The objective of current study was to investigate the association between genetic variation of MCP-3 (rs17735770) and the progression of the disease. Methodology: A case-control study included 50 T2DM patients and 50 healthy controls. DNA was extracted from whole blood samples and used to recognize the MCP-3 gene (rs17735770) polymorphism using conventional polymerase chain reaction (PCR). Results: Concerning the MCP-3 genotypes, there were no statistical differences (P = 0.255) between the studied groups. In patients, the CC and TT frequency genotypes were increased, whereas the control group had a higher ''TC'' genotype frequency. Regarding allele frequency, the “C” allele was higher in patients. On the contrary, the “T” allele was elevated in the control group, with (P=0.390). Conclusion: The current study revealed that the ''CC'' genotype was increased in patients compared to controls. Therefore, the polymorphism of the MCP-3 gene (rs17735770) may represent a risk factor for T2DM.