Healing Effect of Genistein on Detorsioned Rats After Experimental Ovarian Torsion

Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of genistein in preventing damage caused by ovarian torsion.Ovarian torsion is an emergency condition characterized by the complete or partial rotation of the ovary on its supporting ligaments and can affect women of all age groups.Rapid diagnosis is crucial for preserving ovarian function and preventing associated complications.However, due to the relatively nonspecific nature of symptoms, making a diagnosis can be challenging.Methods: In the study, 21 Sprague-Dawley female rats of about four months old, weighing 200-250 g, were used.The study involved three groups of rats: torsion (T), torsion/detorsion (T/DT), and genistein therapy following torsion/detorsion (T/DT/G).Ovarian ischemia was induced in the T and T/DT groups, with the latter undergoing detorsion after 3 hours.The T/DT/G group received genistein treatment (1 mg/kg/day) for 14 days post-detorsion.After treatment, samples were collected for histological and biochemical analysis, including Enzyme-Linked ImmunoSorbent Assay for serum samples and assessment of tissue damage and blood parameters.Results: Our study focuses on genistein, a phytoestrogen described as having similar effects to endogenous estrogen and has gained importance and become an area of interest through advancing research. Conclusion:This study demonstrates that genistein could be an effective protective treatment against post-ovarian torsion damage.However, more comprehensive studies are needed to establish the direct effects of genistein on the ovaries.This study presents important findings supporting the potential clinical use of genistein following ovarian torsion.