Investigating the effect of micromolecules and growth factors on the proliferation of pancreatic beta cells derived from the proliferation of human induced pluripotent cells

Background: Induced stem cell production technology has made diabetic patients more hopeful for alternative treatment.Recently, it has been shown that the use of growth factor micromolecules can play a role in increasing insulin production.Materials and methods: In this study, the effect of growth factors (TGF-, EGF and HGF) and small molecules (harmin and WS6) on insulin production by beta cells was evaluated.Insulin gene expression was evaluated using RT-PCR.Also, the amount of secreted insulin was measured by ELISA.Using the MTT test, the survival rate of beta cells treated with growth factors and micromolecules was evaluated.Results: The results of ELISA and western blot showed that the use of WS6 and harmine along with the simultaneous use of EGH, HGF and TGF- increased protein expression and insulin secretion compared to each alone (p<0.05).Also, the expression of insulin gene was increased according to them.The survival time of the cells treated with growth factors and micromolecules was suitable, and with the increase in their concentration, cell death of beta cells occurred.Conclusion: it can be said that the use of micromolecules and growth factors can have a significant effect on the proliferation of beta cells and insulin production.