Biochemical aspects of the influence of different concentrations of chlorites in drinking water (experimental research)

Objective: determination of the nature and features of changes in biochemical indicators in the blood of animals under conditions of chronic exposure to various concentrations of chlorites in drinking water to substantiate the criteria for hygienic evaluation of their adverse effects on the body. Materials and methods: isolated effect of different concentrations of chlorites in drinking water on biochemical parameters. Results: It was established that the consumption of drinking water with a chlorite content of 0.2 mg/dm3 does not affect the level of biochemical indicators in the blood of experimental animals. Chlorites in concentrations of 0.7 and 1.2 mg/dm3 cause more pronounced changes in metabolic parameters over time. The analysis of the obtained results of studies of integral and specific biochemical indicators shows that with long-term consumption of drinking water with chlorite content in concentrations of 0.7 and 1.2 mg/dm3, an increased content of total protein in blood serum is observed, which may indicate a negative effect of chlorite on processes synthesis. At the same time, compensatory reactions are manifested by shifts in bioenergetic processes and redistribution of metabolic energy-supplying pathways, such as anaerobic glycolysis, with the inclusion of energy resources of creatinine. Load with doses of 0.7 and 1.2 mg/dm3 led to stress at the cellular level, and the mobilization of compensatory mechanisms occurs at the level of metabolism of organ-specific enzymes. It was established that the nature and features of biochemical changes in the blood of experimental animals depended on the concentration of chlorite and the time of action. In the conditions of a chronic experiment, changes in biochemical indicators that do not go beyond physiological deviations of the norm have a stable and permanent character, which is ensured by the development of non-specific protection mechanisms and in this aspect can be considered as adaptive. Conclusions. The nature and peculiarities of changes in biochemical indicators in the body of experimental animals during a six-month chronic sanitary-toxicological experiment with the isolated effect of different concentrations of chlorites in drinking water may indicate a violation of metabolism in organs and changes in the intensity of regenerative processes at the cellular level in response to the adverse effects of toxicants. The established biochemical changes in the blood of the experimental animals are small compared to the control, statistically reliable within the physiological norm and had a constant character, may indicate the development of adaptation processes in the body under the action of chlorites in drinking water. Keywords: drinking water, chlorites, isolated action, biochemical indicators