The Interaction of Lysophosphatidylcholine and Various Tissue-derived Biomaterials on Antimicrobial Effects against Methicillin-resistant <i>Staphylococcus aureus</i>

The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and local wounds continues to be a major global public health concern. Evidence available for the selection of correct antiseptics for preventing and treating wound infections are limited. We here examined whether lysophosphatidylcholine (LPC) remains effective as a wound-compatible antiseptic against in vitro cultivated MRSA in the presence of an organic material. The antimicrobial efficacy of LPC against MRSA was investigated in the presence of various substances, namely bovine serum albumin, defibrinated sheep blood, mucin, and sodium hyaluronate, to evaluate the interactions between LPC and these compounds. We noted the inhibitory effects of 3 mL/L sheep blood and 3 g/L albumin, used to simulate a contaminated wound or environmental surface, on LPC. Two higher concentrations of hyaluronic acid (0.5 and 0.05 g/L) affected LPC activity against MRSA after 3 and 6 h of treatment. In the presence of four concentrations of mucin (30, 3, 0.3, and 0.03 g/L), LPC was almost ineffective against MRSA. We here provided information about interactions between several tissue-derived materials and LPC used against MRSA. In presence of some materials, LPC exhibited relatively stable antimicrobial effect. Thus, LPC may be a promising candidate as a wound antiseptic agent.