Analytical Study of Enzymatic Antioxidants (GPX1, CAT) in Non-Alcoholic Fatty Liver Patients, Relationship to Age and Gender

Enzymatic antioxidants glutathione peroxidase (GPX1) and catalase (CAT) in patients with non-alcoholic fatty liver (NAFLD) reduce the effects of oxidative agents by converting them into water molecules, this study aims to evaluate the accuracy and sensitivity of the analytical method in detecting very low concentrations of GPX1 and CAT and the relationship to NAFLD. The study included males and females with a ratio of 44:46 and after examining them with ultrasound, they were divided into three groups, including the healthy group, the group of patients with light accumulated fat, and the group of patients with medium to severe accumulated. GPX1 and CAT were evaluated by the immunoassay method, lipid levels, fasting sugar, liver enzymes were measured with Cubas 6000 technology. Body mass index (BMI) was calculated by the ratio of weight (kg) to height square in meter (m2). Quantitative results for the lowest concentrations of GPX1 and CAT showed that they were highly accurate, sensitive and repeatable. The statistical significance was high and acceptable for high concentrations in patients for fasting sugar, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and BMI especially in women, either GPX, CAT, aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TG) especially in males, but CAT was not statistically significant (p> 0.05). The results confirmed that the analytical method was highly accurate, and the biomarkers were relevant and influential in NAFLD, the effect of the enzymatic antioxidants GPX1 was more than the CAT that did not show it.