Preliminary study of screen–printed gold electrode for H2O2 sensor based on electrochemiluminescence of luminol

Background: Hydrogen peroxide (H2O2) is mostly used in the water and dairy industries for sterilization and preservation purposes. However, excessive H2O2 residues in milk and tap water pose health risk. Therefore, accurate measurement of H2O2 residue is essential. Methods: This study explores the potential of a screen–printed gold electrode (SPGE) as a sensor for H2O2 sensor using the electrogenerated chemiluminescence (ECL) method of luminol in electrolyte of phosphate buffer solution (PBS) under alkaline condition (pH of 9). Findings: The detection of H2O2 was achieved a linear calibration equation of y = 0.0215[H2O2] + 0.2006 within a concentration range of 0.5 to 200 µM (R2 = 0.9998), demonstrating reliable ECL measurements. Conclusion: The analytical performance evaluation of H2O2 sensor exhibited a low limit of detection (LOD) of 3.06 µM, a limit of quantification (LOQ) of 10.20 µM, and good measurement repeatability, with a relative standard deviation (%RSD) of 6.03%, which is below ⅔ of the Horwitz coefficient of variation (9.85%). Unmodified SPGE offers simplicity, ease of use, a stable surface, and good conductivity while maintaining excellent performance. Novelty/Originality of this article: The application of the ECL method on SPGE for H2O2 detection offers excellent analytical performance, making it a promising approach for monitoring H2O2 residues in the water and dairy industries, with a recovery from 83.83 to 106.01%.