Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L.

This study was carried out to test the effect of BAP (6-Benzylaminopurine) and IAA (Indole-3-acetic acid) hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS (Duchefa Biochemie, Catalog No: M0222) mediums, with BAP or IAA (0.0, 0.5, 1.0, 2.0 mg/l) and BAP+IAA (1.0+1.0, 0.1+2.0, 2.0+0.5 mg/l) added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration (1.7%) in the medium without hormone. The highest success gained for callus regeneration (2.3%) was in the medium having 2.0 mg/l BAP + 0.5 mg/l IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mg/l IAA as 7.7%.