In VIVO MODULATORY EFFECT of MALACHITE GREEN on CYTOCHROME P450 AND ANTIOXIDANT ENZYME ACTIVITIES in RAINBOW TROUT LIVER

Malachite green is a mutagenic and carcinogenic chemical used as a dye in textile and paper industry and in fish farms against ectoparasites, bacterial and fungal infections. Aquatic organisms are at risk of malachite green exposure. In this study, our aim was to determine the effect of two different doses of malachite green on cytochrome P450 system and antioxidant system in rainbow trout (Oncorhynchus mykiss). For this purpose, 24 fish were randomly divided into three groups: the control, the 0.1 mg/L malachite green treatment group and the 0.5 mg/L malachite green treatment group. Highly elevated 7-ethoxyresorufin-O-deethylase activities (EROD) were measured in fish treated with 0.5 mg/L malachite green. 7-pentoxyresorufin O-depentylase (PROD) activities increased with increasing doses of malachite green. Catalase (CAT) and glutathione reductase (GR) activities in the 0.1 mg/L malachite green administration group were higher than those in the control group. Erythromycin N-demethylase (ERND) and glutathione S-transferase (GST) activities were not significantly affected by malachite green treatment in either treatment group. The results of this study clearly demonstrate that malachite green has a modulatory effect on EROD, PROD, CAT, and GR activities in rainbow trout.