Chronic Oral Exposure to V. gracilis Nanoherbs: Toxicity Assessment

The nanoherb Vitis gracilis (Guill. & Perr.) Baker has traditionally been used by farmers to increase their stamina and has recently begun to be scientifically researched by researchers. Here, we analyze the chronic toxicity of V. gracilis nanoherbs and examine their effects on hematological, biochemical, and histopathological conditions of organs. V. gracilis nanoherbs are made with High Energy Milling (HEM) technology. Research treatment with doses of V. gracilis nanoherbs at 100, 200, and 300 mg/kg body weight for 56 days. Mouse blood was then collected to determine mouse blood parameters (hematology), as well as liver and kidney biochemistry associated with various organs, such as histopathology of the liver, kidneys, heart, brain, and lungs. We found that V. gracilis nanoherbs affected histopathological, hematological, and biochemical parameters of organs, at an optimum dose of 100–200 mg/kg body weight. Duration of the grant limitation of this nanoherb is one month. We conclude that the V. gracilis plant may be able to be used as a medicinal herb in the form of nanoherbs in the future, with a maximum dose and period of time of 100–200 mg/kg body weight per day and one month, respectively.