3-Methylpyrazole-Mediated C-Terminal Protein Modification
We present a mild, highly efficient, and selective method for the aminolysis of acyl pyrazole at protein C termini. The protocol demonstrates a broad substrate scope, operational simplicity, and aqueous-compatible conditions. Its successful application to site-specific C-terminal modification and dual functionalization underscores its utility as a versatile platform for protein engineering.
Authors
- Chunmao He (ORCID: https://orcid.org/0000-0003-4596-0032)
- Xinyue Zhang (ORCID: https://orcid.org/0000-0002-5751-7030)
Institutions
- South China University of Technology (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-06-29
- DOI
- https://doi.org/10.1021/acs.orglett.5c02315
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Basic and Applied Basic Research Foundation of Guangdong Province