Determination of Phenolic Content and Biological Activity of Aqueous Extracts of Origanum onites L. from Different Locations

The phenolic content of the decoction and infusion extracts of Origanum onites from three different locations in Trkiye was evaluated using LC-MS/MS.The antioxidant capacity of the decoction and infusion was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), -carotene, linoleic acid, and CUPRAC assays.Additionally, the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of both O. onites extracts were investigated.The highest levels of rosmarinic acid (935.79mg/kg), penduletin (743.53 mg/kg), and kaempferol (506.14 mg/kg) were found in the decoction extract of O. onites from Kemer.In addition, Kemer had the highest DPPH free radical scavenging activity (61.43-75.59%)and lipid peroxidation inhibitory activity (59.03-78.99%) of all the locations examined.Kazda sample had the highest value on the CUPRAC assay (3.83 mmol TR g -1 for decoction and 3.20 mmol TR g -1 for infusion, respectively).The study concludes that a quantitative relationship exists between phenolic content and antioxidant activity, which is important for understanding the role of phenolics in antioxidant activity.The location had an impact on the biological activities of both O. onites extracts, as well as their phenolic content.