Isolation and identification of bacterial endomicrobiome of <i>Syzygium cumini </i>from Faridabad, India

Endomicrobiome is the diverse and dynamic microbial flora that resides in plant tissues, without harming and developing detrimental effects. Syzygium cumini, the black plum or jamun plant, is used as traditional medicine. This is a medicinal plant used against metabolic disorders like diabetes, hypertension, and obesity, etc. The present study aimed at isolating bacterial endomicrobiome from leaf and stem parts of S. cumini locally grown garden situated at 28°20′32″N 77°19′32″E, Faridabad, India. Bacterial endomicrobiome were isolated using nutrient agar plates and identified. The sterilized root and leaf explants were inoculated on the nutrient agar plate and incubated for 24 hours. Morphological, cultural, and staining features were noted for initial identification, and 16S rRNA sequencing to verify the strain. A total of 14 bacterial endomicrobiome isolates were recovered from stem and leaf samples of S. cumini. The leaf sample demonstrated a higher number of bacterial endomicrobiome isolates than the stem of S. cumini. All the obtained isolates were gram-positive, mostly endospore-forming. Morphologically, small rods, creamish, off-white, smooth with irregular margins in appearance while NS5 Bacillus sp. developed slow growing, with brownish colonies on agar medium. Bacillus genera were found as the dominating endomicrobiome of S. cumini. Molecular characterization confirmed that the endomicrobiome of S. cumini was dominated by the genus Bacillus, and Bacillus subtilis was found to be the dominant bacterial species in leaf samples. In contrast, Bacillus safensis was identified as the major bacterial endomicrobiome of S. cumini stems. The obtained sequences were deposited to NCBI under the accession no. Bacillus sp. JL2 PQ475951 and Bacillus sp. NS5 PQ495957. It is concluded that the bacterial endomicrobiome is attributed to the medicinal properties of S. cumini.