EFFECT OF CRUDR AND EXTRACT OF PROPPOLES AS ADDITIVES ON IMMUNOLOGICAL AND BIOCHEMICAL ON CYPRINUS CARPIO CHALLENGED WITH AEROMONAS HYDROPHILA

The present study was carried out to investigate the effect of propolis [(crud propolis and Water Ethanol Extract Propolis (WEEP)] as feed additive on biochemical and immunological in common carp Cyprinus carpio challenged with Aeromonas hydrophila. A total of 60 C. carpio, (75±10g) were randomly stocked into five treated groups in duplicate as follows: control group (C) fish were fed basal diet without any addition of propolis; T1 fish were fed basal diet supplemented with 10g/kg crud propolis; T2, T3 and T4 fish were fed WEEP at concentrations of 2g, 4g and 8 g/kg diet.Results indicated that The highest albumin content was found in T1 which showed a significant increase (P≤0.5) compared to C+ group, and globulin content did not show significant differences between T1, T2, T3, T4, C- and C+ groups. Cholesterol level did not lead to changes in all (P > 0.05) treated groups (T1, T2, T3 and T4) when compared with the control group. The glucose level showed a significant (P≤0.05) increase in all WEEP groups (T2, T3, and T4). The highest weighted Somatic Spleen Index (SSI) was reported in T3 which showed a significant (p < 0.05) increase compared to T1. The result of nitro blue tetrazolium (NBT) assay showed non-significant differences (P > 0.5) in T1, T2, T3 and T4 compared to C and C+ groups.