Biochemical changes in blood and saliva during wound healing in the area of the angle of the mandible in rats with liver cirrhosis

Annotation. Liver dysfunction, characteristic of hepatopathies, is accompanied by cytokine imbalance, oxidative stress and changes in tissue vascularization, which can significantly change the dynamics and efficiency of wound healing. Despite the relevance of this problem, the molecular mechanisms of wound healing in the maxillofacial region against the background of liver pathology are still insufficiently studied, in particular in experimental models. The study of the healing processes of experimental wounds of the maxillofacial region in laboratory animals, such as rats, opens up new opportunities for explaining the pathogenesis and developing effective approaches to treatment of patients with concomitant liver diseases. The studies were conducted on 28 rats, which were subjected to a wound in the area of the angle of the lower jaw. The weight of the rats was 280-320 g. All rats were divided into 4 groups: the first - control group (n = 7) - included rats that were subjected to a wound that healed on its own; the second – experimental 1 – rats were modeled with liver damage (cirrhosis) and a wound was applied; the third – experimental 2 – rats were modeled with liver disease, a wound was applied and arginine glutamate was administered; the fourth – experimental 4 – rats were modeled with liver disease, a wound was applied and arginine glutamate was administered and the wound was lubricated with an Inflarax ointment composition. For all rats, the functional state of the liver was studied by determining the activities of the enzymes alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase in the blood serum, as well as the levels of total protein and ammonia. The state of the oral mucosa was also studied by determining the levels of the pro-inflammatory interleukin-8 and vascular endothelial growth factor in saliva. Our studies have shown that chronic liver damage (cirrhosis of the liver) is accompanied by impaired wound healing in the angle of the mandible, which is closely related to the development of a number of pathochemical changes: the inflammatory reaction and inhibition of the process of vasculogenesis in the oral mucosa, which strongly correlates with signs of liver failure (serum levels of total protein and ammonia, │rs │=0.77-0.86). At the same time, the use of arginine glutamate in combination with inflarax effectively promotes wound healing in the area of the angle of the mandible, due to hepatoprotective activity, the ability to reduce inflammation and stimulate blood vessel growth in the oral mucosa.