Evaluation of hepatoprotective properties of Tagetes Patula L extract in a model of paracetamol-ethanol hepatitis

Annotation. Drug-induced liver damage (DLD) is currently diagnosed in 5-7% of patients admitted to the hepatology department. Pharmacotherapy of DLD with registered drugs with hepatoprotective effects is not always effective, both due to the untimely start of treatment and due to the fact that known drugs cannot affect the main links of pathogenesis. A promising object for reducing the toxic effects of some drugs and ethanol on the liver can be considered a new extract of the herb of the Tagetes Patula L (EНTP), for which antioxidant, anticytolytic, membrane-protective effects have been established in previous studies. The aim of this work was to evaluate the effect of Tagetes Patula L herb extract on changes in clinical and biochemical parameters under conditions of paracetamol-ethanol hepatitis in rats. The studies were performed on a model of subacute paracetamol-ethanol hepatitis in white non-linear male rats, which were administered intragastrically daily for 2 weeks with a paracetamol suspension at a dose of 500 mg/kg and 1 ml of 40% ethanol. Ethanol was administered to potentiate the action of paracetamol. EНTP was administered at a dose of 25 mg/kg 2 weeks before pathology modeling and for 2 weeks during the reproduction of subacute hepatitis. The indicators of bile excretion, the content of bile acids and cholesterol in bile, the concentration of triglycerides in the blood, the activity of alanine aminotransferase and alkaline phosphatase were studied. The results obtained were calculated using descriptive statistics, differences were considered significant at p<0.05. Under the conditions of experimental subacute paracetamol-ethanol hepatitis, the administration of EНTP significantly normalized the bile and bile secretory function of the liver, with an increase of 3.4 times (p<0.05) in bile volume, 3.2 times (p<0.05) in bile secretion rate, 1.6 times (p<0.05) in bile bile acids, and 1.7 times (p<0.05) in bile cholesterol. A powerful anticytolytic and anti-inflammatory effect of EНTP was registered. Silymarin was inferior in effectiveness to the studied extract. According to the studied pharmacological activity, EНTP exceeds the effect of the reference drug Silymarin. Marigold herb extract is a promising object for further preclinical research with the aim of creating an effective herbal preparation based on it for the prevention and treatment of liver lesions, including DLD.