PHARMACOLOGICAL STUDY OF COFFEE-MANGO LEAF EXTRACT COMBINATION AS ANTI-INFLAMMATORY IN RATS

Inflammation occurs as a result of cell-damaging stimuli, causing free arachidonic acid to trigger inflammatory symptoms. Coffee leaves (Coffea arabica) and mango leaves (Mangifera indica) contain phenols, tannins, flavonoids, alkaloids, saponins, terpenoids, and steroids, which have been reported to possess anti-inflammatory activity, giving them great potential and enhancing anti-inflammatory effects when combined. The research aimed to evaluate the anti-inflammatory potential and optimal dosage of a combined extract of coffee and mango leaves (CLMLE). The anti-inflammatory potential was tested through carrageenan induction on the paw of the feet of Sprague Dawley rats in 6 groups of oral doses: negative control (CMC-Na 1%), positive control (Diclofenac Sodium 0.9 mg/200 g BW), CLMLE dose 1 (60 mg/kg BW and 100 mg/kg BW), CLMLE dose 2 (120 mg/kg BW and 200 mg/kg BW), CLMLE dose 3 (240 mg/kg BW and 200 mg/kg BW), and CLMLE dose 4 (120 mg/kg BW and 400 mg/kg BW). The results indicated that all CLMLE doses exhibited significant anti-inflammatory activity compared to the negative control (P < 0.05). Dosage 2 had the highest anti-inflammatory potential, with an inhibition percentage of 76.3431%. The combination of CLMLE in this research was judged to have anti-inflammatory potential, and the findings can be used to produce anti-inflammatory medicines and formulations.