Hyaluronic acid skin booster injection and cold laser therapy in oral wounds: an experimental study

Aim: This study aimed to investigate the impact of combining cold laser therapy with hyaluronic acid skin booster injections on intraoral wound healing, specifically comparing two different incision techniques. Materials and methods: A total of 24 Wistar Albino rats were included in this study and randomly assigned to four groups; two treatment groups and two control groups. In the treatment groups, incisions using blade or 976 nm diode laser were made, followed by cold laser therapy combined with hyaluronic acid skin booster injections. The control groups underwent incisions using either a surgical blade or a 976 nm diode laser (Woodpecker LX16 Plus, Woodpecker, China) without any additional treatment. Tissue samples were collected at baseline and on postoperative days 3 and 7. Histological analysis was performed using Hematoxylin and Eosin staining and Masson’s Trichrome staining to evaluate the wound healing process. Results: Among the experimental groups, the second treatment group demonstrated significantly enhanced wound healing, particularly on days 3 and 7. This group showed increased collagen deposition, better tissue organization, and enhanced angiogenesis compared to the control groups. Conclusion: The combination of cold laser therapy and hyaluronic acid skin booster injections significantly accelerated wound healing following both laser and scalpel incisions. Notably, wounds created using the 976 nm diode laser showed superior healing outcomes compared to those made with a scalpel.