DEVELOPMENT AND VALIDATION OF A UHPLC-DAD METHOD FOR QUANTITATION OF AMYGDALIN IN THE SEEDS OF PRUNUS ARMENIACA L.

In this study, the content of amygdalin in the seeds of Prunus armeniaca L. was quantitatively determined by using a validated UHPLC method. Chromatographic condition was performed on a Halo C18 analytical column (100 × 4.6 mm; 5 μm) with a mobile phase consisting of methanol and water. The gradient elution program was set as follows: 0–40 min, 10–100 % methanol; flow rate was maintained at 0.7 mL/min, and detection was carried out using a UV detector at 207 nm. The calibration curve displayed excellent linearity with an R2 value of 0.9999. The method also showed high sensitivity, with the limit of detection (LOD) and limit of quantification (LOQ) for amygdalin determined to be 0.15 and 0.46 µg/mL, respectively. The chromatographic system exhibited good repeatability with a relative standard deviation (RSD) of 0.07 %. The recovery of method ranged from 100.28 % to 101.41 %, indicating high accuracy and reliability. The results support the potential development of functional foods or nutraceuticals derived from P. armeniaca, where strict control of amygdalin levels is essential.