Biophysical, Biochemical and Functional Characterization of Bioactive Compounds from Tridax procumbens

Medicinal plant products have been used in health care for the treatment of human illnesses since old times.Various homegrown medicinal plants have been used the cure of blood-related issues due to the disturbance of the coagulation pathway.The goal of the study is to characterise the isolated plant leaf extracts of Tridax procumbens that displayed strong anticoagulant properties.The objective of the study is to demonstrate haemolytic activity, wound healing activity, and assessment of the impurity in the partially purified leaf extract from the plant Tridax procumbens.The same fractions were assessed for pro-or anti-angiogenic properties as their therapeutic potential and cellular toxicity for the safety and efficacy of the purified plant extract.The purified leaf extracts contained 31% carbohydrates, 1.1% protein, and 0.9% lipids.The primary active component responsible for the anticoagulant activity is likely to be Azulene, a naphthalene-based derivative.A significant cellular migration was observed, assisting the wound healing.The anti-angiogenic was observed in the purified leaf extracts with no significant cellular toxicity by haemolysis and MTT assay.Traces of protein and lipid sources are present in the purified leaf extract fractions.About 31% of the leaf-extracted fraction has carbohydrates as an active anticoagulant content.The extract showed 90-60% cell migration, suggesting it to be a potent wound healing ability.The purified fractions did not show cytotoxicity at a concentration of 4-500 g/mL in T47D cells.