Algoriphagus aurantiacus sp. nov. and Algoriphagus persicinus sp. nov., two novel species isolated from the shore soil of salt lake

Six Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strains, designated as D3-2-R+10^T, C2-6-M1^T, E1-3-M2, D2-2-M3, D2-2-M2 and D2-2-M1, were isolated from the shore soil of LungmuCo Lake in Xizang Autonomous Region, China. The 16S rRNA gene sequence comparisons confirmed that the six isolates belonged to the genus <i>Algoriphagus</i> of the family <i>Cyclobacteriaceae</i>. The 16S rRNA gene sequence of strains D3-2-R+10^T, D2-2-M3, D2-2-M2 and D2-2-M1 exhibited the highest similarities to the type strains of <i>Algoriphagus antarcticus</i> LMG 21980^T(98.1-98.5 %) and <i>Algoriphagus resistens</i> NH1^T (97.9-98.4 %). Meanwhile, the 16S rRNA gene sequence of strains C2-6-M1^T and E1-3-M2 showed the highest similarities to <i>A. antarcticus</i> LMG 21980^T (98.2-98.7%). The average nt identity and digital DNA-DNA hybridization values among strains D3-2-R+10^T and C2-6-M1^T and their most closely related species <i>A. antarcticus</i> LMG 21980^T were all lower than the threshold values for delineating species, indicating that they represent two separate novel species of <i>Algoriphagus</i>. The predominant cellular fatty acids of strains D3-2-R+10^T and C2-6-M1^T included iso-C_{15 : 0}, summed feature 3 (C_{16 : 1} <i> ω</i>7<i>c</i>/C_{16 : 1} <i> ω6</i>c) and summed feature 9 (iso-C_{17 : 1} <i> ω</i>9<i>c</i>/C_{16 : 0} 10-methyl), and the predominant respiratory quinone was MK-7. The major polar lipids of strain D3-2-R+10^T comprised phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids, while those of strain C2-6-M1^T included phosphatidylethanolamine, two unidentified phospholipids and seven unidentified lipids. The genome size of strains D3-2-R+10^T and C2-6-M1^T was 6.0 and 5.0 Mb, respectively, with the DNA G+C contents of 40.1 and 40.9 mol %. Based on the above descriptions, the six strains were identified as two novel species of the genus <i>Algoriphagus</i>, for which the names <i>Algoriphagus aurantiacus</i> sp. nov. and <i>Algoriphagus persicinus</i> sp. nov. were proposed, with the type strains, respectively.