RNA-seq analysis of transcription patterns during infection of <i>Mesorhizobium japonicum</i> by phage Cp1R7A-A1

RNA-Seq analysis of <i>Mesorhizobium japonicum</i> infected by phage Cp1R7A-A1 (full name vB_MloS_Cp1R7A-A1) revealed the transcription of all 237 putative phage genes, including 27 tRNA and 210 protein-encoding genes. All predicted protein-encoding genes of Cp1R7A_A1 showed expression. The phage tRNA genes also showed a notable expression, indicating a possible tRNA-dependant phage survival strategy. Host genes related to nutrient uptake and energy metabolism were significantly upregulated within 1-2 h post infection, likely reflecting the phage's metabolic demands. Notably, peptidoglycan-cleaving enzymes showed elevated expression after 3 h, suggesting host stress responses. These findings show the transcriptional interplay between phage Cp1R7A-A1 and <i>M. japonicum</i>, providing a foundation for future functional studies.