New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light‐Activatable Bioorthogonal Toolbox
A new photoactivatable bioorthogonal function was added to the photoactivatable bioorthogonal toolbox by transiently disabling the bioorthogonal reactivity of the hydroxylamine moiety with a photolabile protecting group. The photocaged bioorthogonal function was further equipped with a HaloTag substrate to enable its use in live-cell labeling schemes. Light-controlled recovery of reactivity was demonstrated by bioorthogonal fluorescent labeling of intracellular proteins in live cells.
Authors
- Flóra Horváth (ORCID: https://orcid.org/0000-0002-6916-7488)
- Gergely B. Cserép (ORCID: https://orcid.org/0000-0002-0779-0338)
- Péter Kele (ORCID: https://orcid.org/0000-0001-7169-5338)
- Ágnes Szatmári (ORCID: https://orcid.org/0000-0001-8768-8212)
- Krisztina Németh (ORCID: https://orcid.org/0000-0003-1360-7648)
- Evelin Albitz (ORCID: https://orcid.org/0000-0002-3623-0655)
Institutions
- HUN-REN Research Centre for Natural Sciences (HU)
Publication Details
- Journal
- Chemistry - A European Journal
- Published
- 2025-06-27
- DOI
- https://doi.org/10.1002/chem.202501721
- Primary Topic
- Click Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Magyar Tudományos Akadémia
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
- Nemzeti Kutatási, Fejlesztési és Innovaciós Alap