A split ALFA tag-nanobody system for protein localization and proximity proteomics in mycobacteria
This study establishes a new platform for discovery proteomics in mycobacteria using a new nanobody-based approach. The findings will be of interest for all bacteriologists as the approach will be applicable to a variety of microbial systems.
Authors
- Michael S. Glickman (ORCID: https://orcid.org/0000-0001-7918-5164)
- Allison Fay (ORCID: https://orcid.org/0000-0001-5957-5376)
- Jeffrey R. Johnson (ORCID: https://orcid.org/0000-0002-4221-298X)
- Mara Monetti (ORCID: https://orcid.org/0009-0006-9778-9010)
- Zhuoning Li (ORCID: https://orcid.org/0009-0005-9064-8170)
- Andrew P. Kurland
Institutions
- Memorial Sloan Kettering Cancer Center (US)
- Kettering University (US)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- mBio
- Published
- 2025-06-27
- DOI
- https://doi.org/10.1128/mbio.00971-25
- Citations
- 3
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 4.08
Funders
- Instituto Nacional de Cancerología
- National Institute of Allergy and Infectious Diseases