Analysis of Metabolomic Compounds and Antioxidant Potential of Solanaceae Family Plants using Gcms after Elicitor Induction Methyl Jasmonate and Salicylic Acid

Background: Solanaceae plants have the potential to be a source of food, medicine, industrial materials and antioxidants. Antioxidants are compounds that can delay or prevent oxidation caused by free radicals significantly. Metabolomic analysis is a method used to study metabolite profiles. It is necessary to use elicitors to optimize the synthesis of metabolomic compounds and antioxidant potential: methyl jasmonate and salicylic acid as elicitors. Methods: This research aims to analyze the Solanaceae plant’s metabolomic compounds and antioxidant potential using GCMS after the elicitors methyl jasmonate and salicylic acid induction. Experimental method research with stages: 1) Observation, collection and identification of Solanaceae plants, 2) Preparation of experimental land, 3) Planting of plant collections, 4) Application of elicitor methyl jasmonate 60 ppm and salicylic acid 0.45 g/L, 5) Metabolomic and antioxidant analysis on fresh extracts of samples. Testing antioxidant activity using a spectrophotometer with DPPH solution. Metabolomic profile analysis using GCMS. Result: The results of the non-targeted metabolomic analysis with GCMS and antioxidant activity with IC50 in the Solanum genus, namely Solanum betaceum, Solanum melongena, Solanum torvum, Solanum Lycopersicum and Solanum tuberosum, respectively identified 18, 16, 14, 13 and 20 compounds. The IC50 values were 51.32 mg/L (strong category), 56.64 mg/L (strong category), 65.86 mg/L (strong category), 113.60 mg/L (medium category), 102.71 mg/L (medium category), respectively. In the Capsicum genus, namely Capsicum frutescens and Capsicum annuum, 21 and 37 compounds were identified, respectively, with IC50 values of 124.76 mg/L (medium category) and 63.23 mg/L (strong category). In Physalis angulata, 19 compounds were identified, IC50 was 44.04 mg/L (very strong category), six compounds were identified in Nicotiana tabacum and IC50 was 67.55 mg/L (strong category). Solanaceae plants have moderate to very strong antioxidant potential.