Elucidating the Potential of E-cadherin Re-expression along with Trichostatin A and Zebularine in Enhancing Tumour Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis in Human Breast Adenocarcinoma Cells

In summary, H&E staining showed the positive effect of E-cadherin in sustaining apoptosis induced by TRAIL, especially in combination with TSA and Zeb. However, based on flow cytometry, RT-PCR, and Western blot results, TZ and TRAIL could potentially offer a more effective treatment option for E-MDA-MB-231. These findings suggest that TZ induced intrinsic apoptotic pathway via epigenetic modulation of CDH1 promoter while TRAIL-induced extrinsic apoptotic pathway via improved TRAIL signaling with E-cadherin re-ex-pression, indicating the potentiality of E-cadherin as a biomarker for TRAIL treatment in TNBC.