Green Fabrication of Berberine Nano Lipid Carriers by Hot Melt-ultrasonication Method

The purpose of this research was to examine several factors such as lipids, surfactants and their own lipids were the blend of glyceryl monostearate with glyceryl dibehenate (C888), liquid lipid was Miglyol 812N (M812). Non-ionic hydrophilic surfactant-Poloxamer 407 was found to be suitable to provide nanostructure than its counterpart -Tween 80 (Tw 80). The in vitro drug dissolution was following oral administration model, through a dialysis bag, with the media of phosphate buffer pH 6.8, apparatus 2 at the speed of 50 rpm. As a result, NLCs were successfully fabricated from C888, M812 and P407. The value of hydrophilic-lipophilic balance should be from18 to 22 to ensure favourable preparation of BBR-NLCs. 15% liquid lipid over total used lipid (w/w) was shown best BBR encapsulation. The more liquid lipid quantity, the larger the particle size. In general, the best BBR-NLCs had a size of 102.30nm ± 5.49nm, uniform distribution, entrapment efficiency of 85% ± 0.96% and total release of nearly 70% after 24 hours, which was more sustained release than pure drug solution. The stability test proved BBR-NLCs could be considered as stable in nearly 30-day storage at room condition.