The genetic mapping and phenotypic analysis of PatroninF.1.1 in Drosophila melanogaster
The multi-institutional Fly-CURE project is an undergraduate genetics research initiative centered on <i>Drosophila melanogaster</i> as a model organism. This study aimed to characterize and map mutations discovered through a Flp/FRT EMS screen to investigate complex interactions among genes associated with cell division, growth, and apoptosis leading to abnormal cell proliferation. The <i>F.1.1</i> mosaic phenotype resulted in a rough eye phenotype with an overall decrease in representation of mutant tissue. To genetically map the location of the <i>F.1.1</i> mutation, flies with genotype <i>FRT42D,F.1.1, Dark<sup>82</sup> /CyO</i> were crossed with the Bloomington 2R Deficiency Kit. The resultant F1 progeny were analyzed to pinpoint mapping deficiencies. The genomic region containing the <i>Patronin</i> gene was identified and sequencing confirmed the novel allele of <i>Patronin<sup>F.1.1</sup></i> .
Authors
- Jacob D. Kagey (ORCID: https://orcid.org/0000-0002-6994-1795)
- Thais Sholl Guimarães Corrêa
- Kristine Chen
- D. L. Holland
- David W. Walker (ORCID: https://orcid.org/0000-0002-9422-2178)
- Bertha Muñoz Hernández
- Heino Stöver (ORCID: https://orcid.org/0000-0002-2456-2775)
- José L. Gómez (ORCID: https://orcid.org/0000-0002-6521-6318)
- R. Romero (ORCID: https://orcid.org/0000-0002-3573-988X)
- C D C Christie (ORCID: https://orcid.org/0000-0002-8981-3934)
- Alexander Stevens (ORCID: https://orcid.org/0000-0001-7642-0392)
- João Alexandre Cabral (ORCID: https://orcid.org/0000-0002-3333-8898)
- Kayla L Bieser (ORCID: https://orcid.org/0000-0001-5981-2650)
- Danielle R. Hamill (ORCID: https://orcid.org/0000-0002-3748-2643)
- Enhui Hao
- Caroline Sullivan
- L. Gruber
- A. Gerhardt
- Miguel Galícia (ORCID: https://orcid.org/0000-0002-5333-3090)
- Crystal Jara-Pichardo
- Gwyniever M Lonzame
- Abigail Ortiz Olguin
- Spencer G Amacher
- Lillian G Coats
- Claire A Holsted
- Stephen R Kraizel
- Ashley Krumlaw
- Hailey Wyse
- Esmeralda Apodaca
- Brian Arreola
Institutions
- Nevada State University (US)
- Michigan United (US)
- University of Detroit Mercy (US)
- Ohio Wesleyan University (US)
Publication Details
- Journal
- PubMed
- Published
- 2025-03-05
- DOI
- https://doi.org/10.17912/micropub.biology.001564
- Primary Topic
- Developmental Biology and Gene Regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00