Comparative Evaluation of a Disinfectant Formulation Comprising Hydrogen Peroxide, Peracetic Acid, and Acetic Acid Against <i>Aspergillus niger</i> , <i>Escherichia coli</i> , and <i>Staphylococcus aureus</i> on Various Surfaces in Comparison to Othe

This study investigates the effectiveness of a disinfectant formulation comprising acetic acid, hydrogen peroxide, and peracetic acid (AAHPA) against <i>Aspergillus niger</i>, <i>Escherichia coli</i>, and <i>Staphylococcus aureus</i> on petri plates, smooth surfaces, and rough surfaces. Comparative analysis with conventional disinfectants such as 70% isopropyl alcohol, 1.5% chlorhexidine gluconate, 10% sodium hypochlorite, and 0.2% hydrogen peroxide reveal AAHPA's superior performance, achieving significantly higher log reduction (<i>p</i> < 0.05) and percentage reduction (<i>p</i> < 0.01) against <i>A. niger</i> in 5 min on petri plates. On smooth and rough surfaces, AAHPA exhibits exceptional efficacy, demonstrating the highest log and percentage reduction against <i>A. niger</i> in 0.5, 1, and 5 min, surpassing other disinfectants. AAHPA shows the highest bacterial decline against <i>E. coli</i> and <i>S. aureus</i>, followed by 0.2% hydrogen peroxide. In some cases, 0.2% hydrogen peroxide was slightly more effective than AAHPA against <i>E. coli</i> and <i>S. aureus</i> in 5 min. Time-dependent analysis of log reduction trends emphasizes AAHPA's rapid and consistent effectiveness, particularly in 0.5 min, positioning it as a promising disinfectant formulation with broad-spectrum efficacy across diverse surfaces.