Decreased proliferation of HepG2 liver cancer cells in vitro and exhibited proteomic changes in vivo in subjects with metabolic syndrome and metabolic dysfunction-associated steatotic liver disease who performed four-week dawn-to-dusk dry fasting
This study demonstrated that dry-fasted serum collected from subjects with metabolic syndrome and MASLD decreased HepG2 cell proliferation in vitro and showed that proteomic changes occurred in vivo. These findings suggest that DDDF may be an effective intervention for inducing proteomic responses that could assist in the prevention and adjunct treatment of cancers associated with metabolic syndrome.
Authors
- Antone R. Opekun (ORCID: https://orcid.org/0000-0003-4837-6480)
- Ayse L. Mindikoglu (ORCID: https://orcid.org/0000-0003-4598-8692)
- Sung Yun Jung (ORCID: https://orcid.org/0000-0003-1521-7977)
- Mubarak A. Alzubaidi (ORCID: https://orcid.org/0000-0003-3311-3178)
- Kristin L Eckel‐Mahan (ORCID: https://orcid.org/0000-0001-8201-1675)
- Prasun K. Jalal (ORCID: https://orcid.org/0000-0001-5353-2324)
- Zoe R Crochet
Institutions
- Baylor College of Medicine (US)
- The University of Texas Health Science Center (US)
- The University of Texas Health Science Center at Houston (US)
Publication Details
- Journal
- Clinical Proteomics
- Published
- 2025-06-24
- DOI
- https://doi.org/10.1186/s12014-025-09547-3
- Primary Topic
- Dietary Effects on Health
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases